Figure 1.

Binding of normal TPI to IOV. Binding experiments were carried out as described in Materials and Methods. IOV purified from a healthy donor's blood (129 μg) was washed twice and then suspended with the same person's hemolysate (○) or with purified, commercial rabbit muscle TPI (●) solved in buffer A, at 25°C for 30 min. Then, the samples were centrifuged, pellets were resuspended in buffer A, and aliquots were assayed for TPI activity.