Table 3.

Modulation of bindings of TPI and GAPDH to IOVs

Samples	Normal hemolysate, %	Propositus hemolysate, %
TPI	100	100
+ 100 mM KCl	80 ± 10	80 ± 10
+ Antibody	>80	>80
GAPDH	100	100
+ 100 mM KCl	<5	<5
+ Antibody	<5	<20

IOVs were washed twice then resuspended with 700 μl of the corresponding diluted hemolysates (hemoglobin contents: 6.8 g/liter and 3.0 g/liter for propositus and control, respectively). The hemolysates were diluted with buffer A or with buffer A containing 100 mM KCl. The suspensions were incubated for 30 min at 25°C in the presence or absence of 15 μl of antibody raised against the N-terminal sequence of band 3. Then, the samples were analyzed as described in Materials and Methods. The standard error of the determinations was ± 20% (n = 3).