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. 2002 May;76(9):4580–4590. doi: 10.1128/JVI.76.9.4580-4590.2002

FIG. 2.

FIG. 2.

FIG. 2.

Transduction efficiency of adenovirus and AAV vectors in vitro. (A) Slot blot analysis of total DNA from AdlacZ- and AAVlacZ-transduced REC and HeLa cells. Probing for the lacZ reporter gene confirms that cellular viral genome content correlates directly with vector titer. (B) Southern blot of transduced cells probing for the lacZ reporter gene. Total DNA from AdlacZ-transduced REC and HeLa cells reveals a 7,500-bp fragment of adenovirus DNA containing the lacZ gene. AAVlacZ-transduced cells demonstrate multiple genome conformations characteristic of early AAV infection. SS, single-stranded vector genome; M, monomer.