TABLE 2.
Efficiency of plus-strand DNA synthesis as determined by primer extension of chimeric viruses replicated with HHBV P and C proteinsa
| Plus-strand eventb | Steps shown in Fig. 1 | % Efficiency (mean ± SD [P value]) for:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| HHBV | 3E | 5E | M | 3EM | 3E5E | M5E | 3EM5E | ||
| Priming from DR2c | A to C | 67 ± 15 (1.0) | 62 ± 20 (0.6) | 47 ± 14 (0.02) | 40 ± 16 (0.01) | 63 ± 12 (0.6) | 37 ± 12 (0.001) | 73 ± 22 (0.6) | 71 ± 14 (0.7) |
| Priming from DR2 and circularizationd | A to E | 30 ± 4 (1.0) | 25 ± 2 (0.02) | 17 ± 3 (<0.001) | 6 ± 1 (<0.001) | 18 ± 5 (0.001) | 5 ± 2 (<0.001) | 14 ± 5 (0.003) | 34 ± 5 (0.1) |
| Circularizatione | C to E | 45 | 40 | 31 | 15 | 29 | 14 | 19 | 48 |
a
Values are based on measurements made with intracellular viral DNA isolated from the following numbers of independent transfections: for HHBV, 7; for 3E, 5; for 5E, M, 3EM, 3E5E, M5E, and 3EM5E, 6. Two-sample separate variance t test was used to detect differences in means between HHBV and mutant values for priming from DR2 and for priming from DR2 and circularization. Statistical tests were carried out with Systat software.
b
See Materials and Methods for an explanation.
c
B/C value.
d
A/C value.
e
A/B value.