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. 2000 Feb 1;97(3):1032–1037. doi: 10.1073/pnas.97.3.1032

Figure 5.

Figure 5

Ligand-independent degradation of immature PRs. (A) Immature PRs are rapidly degraded in the absence of ligand. T47D-YB cells were treated without [C (EtOH vehicle)] or with R5020 alone [R (10 nM)] or with the hsp 90-binding agent GA (G) alone (10 μg/ml) for 2, 4, 6, and 8 h, and PR-B protein in whole-cell lysates was measured by immunoblot analysis. (B) Ligand-independent degradation is mediated by the 26S proteasome. Duplicate cultures of T47D-YB cells were treated as indicated, without [C (EtOH vehicle)] or with R5020 [R (10 nM)] alone, GA [G (10 μg/ml)] alone, or with GA plus lactacystin [Lt. (10 nM; 30-min pretreatment)], GA plus R5020, or lactacystin alone for 6 h. PR-B protein in whole-cell lysates (100 μg of protein per lane) was measured by immunoblot analysis with PR-specific antisera.