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. 1999 Feb 16;96(4):1547–1552. doi: 10.1073/pnas.96.4.1547

Figure 3.

Figure 3

Comodulation of unstimulated TCRs upon down-regulation of specific TCR/CD3 complexes in dual-receptor T cells. Immunofluorescence distribution profiles of spleen T cell lines from Vβ2×Vβ8 double transgenic mice submitted to different TCR down-regulation protocols and stained with FITC-labeled mAb against TCRVβ8 (A) or TCRVβ2 (B). Left histograms in both A and B represent the background staining with anti-Ig fluoresceinated control reagents that was superimposable to the autofluorescence (data not shown). Shaded histograms show the comodulation of the stained, unstimulated TCRs promoted after down-regulation of the reciprocal TCRβ, done by incubation at 37°C for 4 h in plates coated with 5 μg/ml of mAb specific for the reciprocal TCRVβ. Down-modulation is not promoted either in replicate plates kept in parallel at 4°C (dotted line) or by a control mAb [OKT3, which promotes TCR/CD3 comodulation in human CD3 transgenic T cells (10)] (continuous line), as shown in the right histograms. The mAbs do not promote modulation of the reciprocal TCRβ in single transgenic mice (data not shown). Results are representative of four experiments.