FIG. 4.

Yeast two-hybrid interactions between gE and gM and the UL49 protein. (A) Schematic depiction of the principles of the two different yeast two-hybrid systems used. min., minimal. (B) Observed interactions. Bars represent proteins with hydrophobic signal sequences (for gE) and transmembrane domains (for gE and gM), highlighted by grey shading. Also indicated are the different portions of gE, gM, and UL49 found in the interacting fusion proteins from the PrV expression libraries. Proteins from PrV-Be (gE-Be and UL49-Be) and PrV-Ka (gM-Ka, UL49-Ka, and gE-Ka) were analyzed.