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. 2002 Aug;76(16):8058–8068. doi: 10.1128/JVI.76.16.8058-8068.2002

FIG. 1.

FIG. 1.

Plasmid constructions. (a) pIV1003 contains the first 416 nt of the HCV genotype 1a genome (clone p90/HCV FL-long pU) directly under the control of the T7 promoter. (b) pIV1033 contains the HIV TAR (2) sequence cloned in front of nt 1 of the HCV genome in pIV1003. (c) Sequence details of the junction between the extremity of the HCV terminus and the nucleotides of the HIV-1 TAR sequence that base-pair with the 3′ end of the HCV RNA. HCV nucleotides are in the shaded box (in italics and boldface), and HIV nucleotides are in boldface; an extra U (circled) was added by cloning.