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. 2002 Aug;76(16):8058–8068. doi: 10.1128/JVI.76.16.8058-8068.2002

FIG. 4.

FIG. 4.

Probing experiments on nt −1 to −106 (domain I; SL-AI and SL-BI). (a) Modifications of U(N-3) and G(N-1) with CMCT. Lane 1, incubation control; lane 2, 15 min; lane 3, 30 min; lane 4, 45 min. (b) Modifications of A (N-1) and C(N-3) with DMS. Lane 1, incubation control; lane 2, 5 min; lane 3, 10 min; lane 4, 15 min; lane 5, 20 min. (c) Cleavage of double-stranded and stacked RNA regions by RNase V1. Incubation was with 0.035 U per reaction at 20°C. Lane 1, incubation control; lane 2, 5 min; lane 3, 10 min; lane 4, 15 min. (d) Cleavages of accessible RNA regions by lead. Incubation was for 5 min at 20°C. Lane 1, incubation control; lane 2, 12 mM lead acetate; lane 3, 40 mM lead acetate; lane 4, 120 mM lead acetate. Sequencing reactions (U, G, C, and A) were run in parallel.