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Probing experiments on nt −104 to −288 (domain I, SL-CI, SL-DI, and SL-EI; domain II, SL-AII). Cleavages with RNase V1 (a and b) and lead (c) were as for Fig. 4. (d) Modifications with CMCT and DMS. Lane 1, incubation control with buffer M1; lanes 2 to 5, incubation with DMS for 5 (lane 2), 10 (lane 3), 15 (lane 4), and 20 min (lane 5); lane 6, incubation control with buffer M2; lanes 7 to 9, incubation with CMCT for 15 (lane 7), 30 (lane 8), and 45 min (lane 9).
