FIG. 8.

Cellular PKC activity does not mediate HBV core protein phosphorylation. Total cell lysates from HuH-7 cells were incubated with either dimethyl sulfoxide or the indicated amounts of bisindolylmaleimide I (Bis-1). Aliquots were then added to kinase reaction mixes containing the same concentrations of bisindolylmaleimide I, which were supplemented with calcium, phosphatidylserine (PS), and diacylglycerol (DAG) where indicated. Kinase reactions were performed in the presence of either histone H1 (upper panel) or GST-HBV-C1 protein (lower panel). After gel electrophoresis, substrate phosphorylation was visualized by autoradiography. Positions of kinase substrates are indicated on the right.