TABLE 2.
Sequence analysis of SSH clones selected by hybridization screening
| Identification (GenBank, accession no.) | No. of clones | Other similar sequence(s) | RT-PCR assay result |
|---|---|---|---|
| A29 (AF483529) | 21 | Ubiquitin-like (O. mykissAF206323, E = 2e − 40; H. sapiensAB003730E = 6e − 25)a | + |
| vig-2 (AF290477) | 14 | Vig-2 (O. mykissAF290477, E = 0) | + |
| B44 | 11 | Globin (O. mykissD82926, E = 3c − 24, blastn) | − |
| B6 (AF483530) | 7 | Retinoic acid and interferon-induced protein (H. sapiens U34605, E = 7e − 11) | + |
| A83 (AF483533) | 6 | Galectin-9 (H. sapiensAJ288083, E = 0.0034; O. mykissAB027462, E = 5e − 03) | + |
| A14 (AF483527) | 5 | CXC chemokine (H. sapiens P19875, E = 7e − 5) | + |
| B32 (AF483536) | 5 | Estrogen responsive protein (H. sapiens D21205, E = 7e − 7) | + |
| B143 (AF483539) | 4 | + | |
| B305 (AF483542) | 3 | + | |
| B126 (AF483532) | 3 | Hypothetical protein (Drosophila T13747, E = 2e − 12) | + |
| vig-1 (AF076620) | 2 | vig1 (O. mykissAF076620, E = 0.0) | + |
| mx-3 (U47946) | 2 | mx3 (O. mykissU47946, E = 0.0) | + |
| B153 (AF483547) | 2 | + | |
| A27 | 1 | Alu repeat | ND |
| B322 | 1 | ImmunoglobulinM (Salmo salar, Y12391E = 9e − 10, blastn) | ND |
| B68 (AF483528) | 1 | CXC chemokine (S. scrofulX77935, E = 0.072; H. sapiensU59286, E = 0.8) | + |
| B17 (AF483531) | 1 | Interferon-responsive protein (H. sapiensAJ312776, E = 0.007) | + |
| B225 (AF483540) | 1 | + | |
| B12 (AF483534) | 1 | Hypothetical protein (H. sapiensAK022542, E = 5e − 13) | + |
| B203 (AF483538) | 1 | uvrD-helicase domain containing protein (H. sapiens emb/CAC15527, E = 4e − 13) | + |
| B88 (AF483541) | 1 | + | |
| B51 (AF483537) | 1 | Coatomer protein complex (H. sapiensAK003458, E = 2e − 41) | + |
| B319 (AF483543) | 1 | + | |
| B21 (AF483544) | 1 | + | |
| B160 (AF483545) | 1 | + | |
| B124 (AF483546) | 1 | + | |
| B324 | 1 | Ribosomal protein (H. sapiensXM_096067, E = 1 − 13) | − |
| B191 (AF483535) | 1 | C-lectin (O. mykissAF281349, E = 6e − 04) | + |
a
Accession numbers, name of the species, and E values of Blast hits are indicated. E values correspond to the BlastX program, except when “Blastn” is specified.