TABLE 3.
Genes modulated by VHSV
| Gene | Stimulation indexa | Protein family/ most significant hits and E values for BlastX program | Potential function(s) |
|---|---|---|---|
| vig-3 (clone A29) | 7.2 | Ubiquitin-like protein (Carassius auratus AF206323, E = 2e − 40; H. sapiens ISG15 NM_0051017, E = 7e − 18) | Interferon induced (ISG15); human homolog blocked by influenza virus |
| vig-4 (clone B6) | 1.9 | Retinoic acid- and interferon-inducible protein with tetratricopepetide domain (H. sapiens NM_012420 (E = 7e − 655)) | Interferon induced (p58/p56); myeloid differenciation marker, interaction with eukaryotic initiation factor (translation regulation), glucocorticoid attenuated response |
| vig-5 (clone B17) | 2.4 | Transmembrane protein 7 (H. sapiens NM_031440 E = 2e − 22) | Interferon induced (p28) Unknown function |
| vig-6 (clone B126) | 2.6 | Protein with six transmembrane regions (Mus musculus AK015888, E = 5e − 44; H. sapiens XM_059238, E = 2e − 41, Drosophila melanogaster AAF45721, E = 2e − 29); Hs and Dm promoters have ISRE | Interferon induced (?) Unknown function |
| vig-7 (clone A14) | 2 | CXC chemokine (L. fluviatilis IL-8 AJ231072 E = 1e − 11 and other CXC) | Chemoattractant |
| vig-8 (clone B68) | 1.6 | CXC chemokine (S. scrofa PDPP X77935, E = 6e − 12 and other CXC) | Chemoattractant |
| vig-9 (cloneA83) | 2.6 | Galectin-like protein (O. mykiss AB027452, E = 2e − 92) galectin-9 Mus musculus U55061, E = 2e − 59) | Chemoattractant, apoptosis |
| vig-10 (clone B12) | 1.6 | Unknown protein with poly ADP-ribose polymerase catalytic domain (H. sapiens AK022542, E = 2e − 59) | Transcription repression, apoptosis |
| clone B203 (partial cDNA) | 2.1 | Unknown nucleic acid binding protein with ribonuclease and uvr-helicase domains (H. sapiens XM_028918 (KIAA1769), E = 1e − 104) | RNA metabolism |
a
Stimulation index (S) was calculated as follows: from quantification of Northern blots using a PhosphorImager: S = (Vs/ Vact)/(Cs/Cact), where Vs and Vact correspond to the signal obtained for the virus-treated RNA hybridized with specific and actin probe, respectively; Cs and Cact correspond to the signal obtained for the control RNA hybridized with specific and actin probe, respectively.