FIG. 6.

Localization of p16 at different times of infection. BHK cells (A) or HeLa cells (B) were grown on coverslips and infected with MVA at an MOI of 10. The cells were fixed at the indicated times postinfection (pi.) and double labeled with DAPI (a, c, e, and g) and anti-p16 (A14L) followed by anti-rabbit antibody coupled to rhodamine (b, d, f, and h). Note that p16 perfectly overlaps with the regions labeled with DAPI in BHK cells infected with MVA from 5 h 30 min postinfection onwards. p16 does not overlap to the same extent in HeLa cells infected with MVA. At 4 h 30 min postinfection under both permissive and nonpermissive conditions, p16-positive spots that do not colocalize with the viral DNA are detected.