TABLE 1.
Summary of data obtained from IL-3 independence growth assays
| Ba/F3 cell genotype | No. of positive cell lines/total no. of independently derived cell lines tested | Growth phenotypea | Ba/F3 cell genotype | No. of positive cell lines/total no. of independently derived cell lines tested | Growth phenotype | |
|---|---|---|---|---|---|---|
| LXSN | 0/21 | − | PTF | 0/2 | − | |
| E5 | 0/21 | − | PTF + E5 | 0/2 | − | |
| v-sis | 0/20 | − | PTF + v-sis | 2/2 | + | |
| PR | 1b/23 | − | ERTM | 5b/13 | − | |
| PR + E5 | 23/23 | + | ERTM + E5 | 3b/13 | − | |
| PR + v-sis | 22/22 | + | ERTM + v-sis | 12/12 | + | |
| PRSpeI | 0/3 | − | ERTML513T | 2b/12 | − | |
| PRSpeI + E5 | 3/3 | + | ERTML513T + E5 | 5c/12; 2b/12 | +/− | |
| PRSpeI + v-sis | 3/3 | + | ERTML513T + v-sis | 11/11 | + | |
| V500P | 0/3 | − | ERTML513TSpeI | 0/3 | − | |
| V500P + E5 | 2/3 | + | ERTML513TSpeI + E5 | 2c/3 | +/− | |
| V500P + v-sis | 3/3 | + | ERTML513TSpeI + v-sis | 3/3 | + | |
| V500L | 0/2 | − | PR/ERL513T | 1b/3 | − | |
| V500L + E5 | 2/2 | + | PR/ERL513T + E5 | 1b/3 | − | |
| V500L + v-sis | 2/2 | + | PR/ERL513T + v-sis | 2/2 | + | |
| V501L | 0/2 | − | ER/PR | 0/2 | − | |
| V501L + E5 | 2/2 | + | ER/PR + E5 | 0/2 | − | |
| V501L + v-sis | 2/2 | + | ER/PR + v-sis | 2/2 | + | |
| V502T | 0/3 | − | NNTM | 1b/3 | − | |
| V502T + E5 | 3c/3 | +/− | NNTM + E5 | 1b/3 | − | |
| V502T + v-sis | 3/3 | + | NNTM + v-sis | 3/3 | + | |
| V502L | 0/2 | − | NNTML513T | 1b/3 | − | |
| V502L + E5 | 2/2 | + | NNTML513T + E5 | 1b/3 | − | |
| V502L + v-sis | 2/2 | + | NNTML513T + v-sis | 3/3 | + | |
| I503F | 0/3 | − | NNTML513TSpeI | 0/2 | − | |
| I503F + E5 | 3c/3 | +/− | NNTML513TSpeI + E5 | 0/2 | − | |
| I503F + v-sis | 3/3 | + | NNTML513TSpeI + v-sis | 2/2 | + | |
| I503L | 0/2 | − | PR/NRL513T | 0/2 | − | |
| I503L + E5 | 2/2 | + | PR/NRL513T + E5 | 0/2 | − | |
| I503L + v-sis | 2/2 | + | PR/NRL513T + v-sis | 2/2 | + | |
| S504I | 0/2 | − | NR/PR | 0/2 | − | |
| S504I + E5 | 2c/2 | +/− | NR/PR + E5 | 0/2 | − | |
| S504I + v-sis | 2/2 | + | NR/PR + v-sis | 2/2 | + |
+ and − indicate ability or inability, respectively, to grow in the absence of IL-3. An IL-3-independent (+) growth phenotype was assigned to cells that could consistently proliferate in the absence of IL-3 20-fold over a period of 10 days. +/− was assigned to cells that inconsistently displayed an intermediate IL-3-independent growth phenotype, as described below.
Considered background growth if growth was slowed, the saturation density attained was suboptimal, and cells not expressing E5 or v-sis established in parallel grew in a similar manner.
Considered intermediate growth because proliferation was significantly delayed and the saturation density attained was 8 to 20 times the seeded density.