FIG. 2.

Distribution of μNS(T1L) and β-tubulin in transfected cells with and without nocodazole treatment. CV-1 cells transfected with 2 μg of pCI-M3(T1L) per well were left untreated (upper panels), treated with 10 μM nocodazole added at 6 h p.t. (middle panels), or treated with 10 μM nocodazole added at 17 h p.t. (lower panels). The cells were fixed at 18 h p.t. and immunostained with Oregon green-conjugated anti-μNS rabbit IgG (red) (first column) and Cy3-conjugated mouse monoclonal antibody to β-tubulin (green) (second column). Nuclei were counterstained with DAPI (blue). The boxed area in the merged image (third column) is enlarged to show detail (inset). Bars, 10 μm.