FIG. 3.

Colocalization of μNS(T1L) and μ2 in cotransfected cells. (A) CV-1 cells were cotransfected with 1 μg of pCI-M3(T1L) and 1 μg of pCI-M1(T1L) (upper panels) or 1 μg of pCI-M3(T1L) and 1 μg of pCI-M1(T3D^N) (lower panels) per well and fixed at 18 h p.t. Cells were immunostained with Texas red-conjugated anti-μNS rabbit IgG (red) (first column) and Oregon green-conjugated μ2 rabbit IgG (green) (second column). Nuclei were counterstained with DAPI (blue). (B) CV-1 cells were transfected with 2 μg of pCI-M1(T1L) (left) or pCI-M1(T3D^N) (middle and right) per well, fixed at 18 h p.t., and stained with rabbit anti-μ2 polyclonal serum and goat anti-rabbit IgG conjugated to Alexa 488. Bars, 10 μm.