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Colocalization of μNS(T1L) and μ2 in cotransfected cells treated with nocodazole. CV-1 cells were cotransfected with 1.75 μg of pCI-M3(T1L) and 0.25 μg of pCI-M1(T1L) (upper four panels) or 0.25 μg of pCI-M1(T3DN) (lower four panels) per well. Cells were left untreated or treated with 10 μM nocodazole added at 6 h p.t. (nocodazole), fixed at 18 h p.t., and immunostained for μNS (left column) and μ2 (right column) as described in the legend to Fig. 3A. Bars, 10 μm.
