FIG. 7.
Distribution of μNS(41-721) in transfected cells with and without μ2 coexpression. (A) CV-1 cells were transfected with 1 μg of pCI-M3(T1L) or pCI-M3(41-721) and 1 μg of pCI-neo as a carrier plasmid per well. CV-1 cells were infected (inf) with T1L at a multiplicity of infection of 5. Lysates were collected at 18 h p.t. or p.i. and analyzed by SDS-PAGE and immunoblotting with polyclonal antisera specific for full-length μNS (α-μNS) and the N-terminal 41 amino acids of μNS (α-1-41 μNS). A band appearing in untransfected CV-1 cells is indicated on the right with an asterisk. The position of full-length μNS is indicated on the right. (B) Phase-contrast microscopy (left) and IF microscopy (right) of CV-1 cells transfected with 2 μg of pCI-M3(41-721) per well. Cells were fixed at 18 h p.t. and immunostained with Texas red-conjugated anti-μNS rabbit IgG. (C) CV-1 cells were cotransfected with 1 μg of pCI-M3(41-721) and 1 μg of pCI-M1(T1L) (upper panels) or 1 μg of pCI-M3(41-721) and 1 μg of pCI-M1(T3DN) (lower panels) per well and fixed at 18 h p.t. Cells were immunostained for μNS (red) and μ2 (green) as described in the legend to Fig. 3A. Bars, 10 μm.