FIG. 7.

Effect of BZLF1 on LMP1-mediated activation of NF-κB. (A) Dose-dependent inhibition of NF-κB activation by BZLF1. As much as 6 μg of a BZLF1 expression plasmid was cotransfected with 3 μg of the NF-κB reporter 3Enh-Luc in DG75 cells. The total amount of DNA per transfection was kept to a constant 9 μg by addition of an appropriate amount of empty vector DNA. Luciferase activity was measured after 24 h. Results shown are means of results from duplicate experiments. (B) Effect of BZLF1 on LMP1-mediated NF-κB activation. DG75 cells were cotransfected with 3 μg of the NF-κB reporter 3Enh-Luc together with either 4 μg of pSG5 empty vector DNA, 4 μg of an LMP1 expression plasmid, 4 μg each of an LMP1 expression plasmid and a BZLF1 expression plasmid, or 4 μg each of an LMP1 expression plasmid and an IκBαΔN expression plasmid, exactly as for Fig. 6A. The total amount of DNA per transfection was kept constant by addition of an appropriate amount of empty vector DNA. Luciferase activity was measured after 24 h. Results shown are means and standard errors from triplicate experiments.