FIG. 6.
Anti-IFN-γ blocks CD8+ T-cell help. Vaccine-primed CD8+ T cells were purified by positive selection and transferred to naive H-2a/a mice as described for Fig. 1. One day posttransfer, mice were infected with 15 SFFU of FV-B. Mice were then given 0.5 ml of either anti-IFN-γ Cellmax supernatant or rat Ig every other day starting on the day of infection. Alternatively, one group of H-2a/a mice were given 105 U of IFN-γ intraperitoneally on the day before infection, the day of infection, and on days 2, 4, and 6 postinfection. At 8 days postinfection, CD4+ T cells were purified and analyzed for IFN-γ production as described for Fig. 1. Data shown are the average values + SEM for eight mice per group and are the combined results from two separate experiments.