TABLE 1.

FV-specific CTL responses in H-2^a/a mice after vaccination with FV-N^a

Infectious agent	No. of CTL-positiveb mice/total no. tested for:
	AA41 (KkDd) cells	D1B (KdDd) cells
FV-B	0/8	0/8
FV-N	10/22	9/22

^aH-2^a/a mice [(B10.A × A/WySn)F₁] were infected with 100 SFFU of FV-B or FV-N. At 14 to 21 days postinfection, spleen cells were used in a direct CTL assay with ⁵¹Cr-labelled target cells at a ratio of 200:1 (spleen cells/target cells). Assay mixtures were incubated for 19 h at 34°C (D1B target cells) or for 5 h at 37°C (AA41 target cells) as previously described (6). No lysis was observed when Y57-2C (H-2^b) FV-transformed cells were used as targets for these H-2^a/a mice in a 5-h, 37°C assay.

^bCTL responses were determined for AA41 and D1B target cells. See reference 6 for details.