FIG. 2.

Detection of AL-RNA by RT-PCR. PC-12 cells were infected at an MOI of 5. Total RNA was isolated 6 h p.i., and RT-PCR was performed as described in Materials and Methods. Lanes: 1, no RT, wild-type McKrae virus-infected PC-12 cells; 2, wild-type McKrae virus; 3, dLAT2903R; 4, dLAT2903; 5, uninfected PC-12; 6, no RT, dLAT2903; 7, no RT, uninfected PC-12; 8, marker, PCR of wild-type McKrae viral DNA.