FIG. 3.

Mapping the 3′ end of the AL-RNA. RT-PCR was performed on poly(A)⁺ RNA isolated from PC-12 cells infected with wild-type McKrae virus, and the RT-PCR product was cloned and sequenced as described in Materials and Methods. The resulting sequence is labeled RNA. The “genome” sequence is from the same HSV-1 strain (McKrae). The asterisks in the “genome” sequence indicate identity to the RNA sequence. This identity ends after genomic nucleotide 118603. The positions of the 5′ primer and the 3′ oligo(dT) primer are shown.