FIG. 3.

Degradation of class I and II proteins by US2 C- and N-terminal truncation mutants. His16 cells were infected for 12 to 16 h with Adtet-trans alone or coinfected with Adtet-trans (Trans) and AdtetUS2 (wild-type US2 [US2wt]) or Ad vectors expressing US2 mutants: 1-186, 1-160, 1-150, 1-140, 1-130, 1-120, or 1-110 (A) or K^b21-199, K^b28-199, or K^b40-199 (B). The cells were radiolabeled with ³⁵S-labeled methionine and cysteine for 1 min, and then the label was chased for 15 or 30 min. The cell extracts were immunoprecipitated with MAb HC10 (anti-class I HC, upper panels) or DA6.147 (anti-class II DR-α, lower panels).