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. 2002 Aug;76(16):8265–8275. doi: 10.1128/JVI.76.16.8265-8275.2002

FIG. 6.

FIG. 6.

Expression of US2/3 chimeric proteins and degradation of class I and II proteins. (A) Schematic representation of US2 and US3 including the homologous sequences: hr1, hr2, and hr3. US2/3-hr1 contains hr1 of US3 introduced into US2 in place of US2 hr1. US2/3-hr2 has hr2 of US3 introduced into US2 in place of US2 hr2. US2Δhr3 has a deletion of 4 aa (RCVP) in the hr3 region. (B) Expression of US2/3 chimeric proteins. His16 cells were infected with Ad vectors, the cells were radiolabeled for 5 min with 35S-labeled methionine and cysteine, and US2/3 proteins were immunoprecipitated with anti-US2 antibodies. (C) Degradation of class I and II proteins by US2/3 chimeric proteins. His16 cells were infected with Ad vectors and labeled with 35S-labeled methionine and cysteine for 1 min, and then the label chased for 15 or 30 min. MHC class I HC (upper panel or class II complexes (lower panel) were immunoprecipitated with MAb HC10 or DA6.147, respectively.