FIG. 3.

Confocal micrograph of wild-type-infected optic nerves. The rat visual system was infected with wild-type virus, and 35-μm-diameter sections were prepared and incubated with antibody against PRV virion proteins (in green) (A) and an antibody against the astroglial cell marker GFAP (in red) (B). Secondary antibodies were then used to visualize where primary antibody had bound, and confocal microscopy was used to capture images. The merged image is shown in panel C. The circled nonneuronal cell is immunopositive for both virion proteins and GFAP, whereas the arrow points to a nonneuronal cell that is not GFAP positive. Bar, 25 μm.