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. 2002 Aug;76(16):8468–8471. doi: 10.1128/JVI.76.16.8468-8471.2002

FIG. 2.

FIG. 2.

Replication of VZV rOka, rOkaΔgI, and rOka-gI@Avr in human skin. (A) Six implants infected with rOka or rOka-gI@Avr were harvested at 10 days postinfection, and six implants infected with rOkaΔgI were harvested at 21 days postinfection. The number of PFU/implant was determined in an infectious focus assay. Each point represents one human skin implant, and the bar indicates the average PFU/implant. Where points overlap, the number of implants analyzed is given in parentheses. (B) Immunoblot analysis of three skin implants infected with rOka or rOka-gI@Avr and six implants infected with rOkaΔgI. VZV proteins in the 70- to 110-kDa range were detected with high-titer human polyclonal antiserum. (C) PCR analysis of the gI region of viruses recovered from skin implants. Primers spanning the AvrII restriction site, gI, or β-actin were used, and the sizes of the fragments produced are given in kilobases (center). These results represent similar data obtained from three separate experiments.