FIG. 7.

Lower activity of a Rev deletion mutant. HeLa cells were transfected with pDM128 and pCDMβ-Gal together with various amounts of pRev-VP or pRev86-VP. CAT/β-Gal values were calculated. The results are presented as n-fold activation relative to the basal CAT/β-Gal value obtained by transfection of pDM128 and pCDMβ-Gal without any effector plasmids. The results represent the means and standard deviations of three independent experiments. Western blotting and the quantitation data under the graph indicate the levels of the VP16-fused protein expression in this functional assay in which 0.01 μg and 0.2 μg of VP16-fused Rev derivative expression plasmids were transfected. In the quantitation data, the levels of Rev86-VP expression relative to that of Rev-VP were calculated.