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. 2002 Aug;76(16):8079–8089. doi: 10.1128/JVI.76.16.8079-8089.2002

FIG. 8.

FIG. 8.

Lower expression of Gag from an HIV-1 proviral plasmid harboring the C-terminally truncated Rev mutant. HeLa cells were transfected with pNL4-3LucE−R−(Rev) or pNL4-3LucE−R−(Rev86). (A) At 48 h posttransfection the supernatants were subjected to p24 ELISA. At the same time the cells were lysed and Luc activity was measured to monitor the efficiency of transfection. p24 and Luc values were calculated. The results are shown as the means with standard deviations of several experiments. (B) The normalized volume of cell lysates was subjected to Western blot analysis to detect intracellular levels of p24. The mock sample received pSRα296 alone. (C) The expression of Rev by HIV-1 proviral plasmids in the presence of a dominant negative RevM10. HeLa cells were transfected with 1.0 μg of each HIV-1 proviral plasmid along with various amounts of pGAL-RevM10. At 48 h posttransfection, the cells were lysed and subjected to Western blot analysis. 8E7 anti-Rev monoclonal antibody was used as the primary antibody.