Figure 5.

Depletion of mitochondrial Yah1p causes a defect in the incorporation of an Fe/S cluster into the cytosolic Fe/S protein Leu1p. (A) Wild-type (WT), Gal-YAH1, Δcox6, and Δcor1 cells were grown in iron-free minimal medium for the indicated times. Radiolabeling, cell lysis, and analysis of ⁵⁵Fe incorporation into cytosolic Leu1p by immunoprecipitation was performed as described for Fig. 3B. (B) The uptake of radioactive iron into the various yeast cells grown for 64 h in minimal medium was quantitated by liquid scintillation counting from the amount of ⁵⁵Fe in the extracts.