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. 2000 Feb 1;97(3):1056–1061. doi: 10.1073/pnas.97.3.1056

Figure 4.

Figure 4

HDRP interacts with both HDAC1 and HDAC3. FLAG-HDRP was immunoprecipitated from transfected Cos7 lysates by using anti-FLAG antibody, resolved by SDS/PAGE, and subjected to Western blot analyses. (A) Cos7 cells were transfected with vector alone (lanes 1 and 5), pFLAG-HDRP alone (lanes 2 and 6), pFLAG-HDRP with pHA-HDAC1 (lanes 3 and 7), or pHA-HDAC1 alone (lanes 4 and 8). Lanes 1–4, whole-cell lysates; lanes 5–8, anti-FLAG IP; lanes 9 and 10, anti-FLAG IP in the presence of FLAG-peptide for pFLAG-HDRP alone and for pFLAG-HDRP with pHA-HDAC1, respectively. (Upper) Anti-HA antibody was used for Western blotting; (Lower) anti-FLAG-M2 antibody was used for Western blotting. (B) The samples shown were described for A, except that pCMV-HDAC3 replaces pHA-HDAC1 in the transfection and that anti-HDAC3 antibody was used for Western blotting in Upper.