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. 2000 Feb 1;97(3):1056–1061. doi: 10.1073/pnas.97.3.1056

Figure 6.

Figure 6

Gal4-HDRP represses activated transcription. Gal4-LexA-E1B-Luc was transfected into Cos7 cells with 10 ng of pCMV-LexA (1–202) (−) or with 10 ng of pCMV-LexA-VP16 (+) along with the indicated amount of pGal4-HDRP. pCMV-Gal4 (1–147) was included in experimental groups with pCMV-LexA (1–202) or pCMV-LexA-VP16 alone. Results are shown as the means of three independent transfections ± standard deviation. The Fluc activity was first normalized to the cotransfected Rluc activity. The VP16 activated value was set to 1. VP16 activated transcription 238-fold compared with the basal level.