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. 2006 Aug 21;103(35):13180–13185. doi: 10.1073/pnas.0605669103

Fig. 3.

Fig. 3.

EMT develops in vivo after TGF-β1-mediated lung injury. (A and B) The same field (×60) of a lung 3 weeks after intranasal AdTGF-β1 stained by X-gal (B) then immunostained (A) for α-SMA and pro-SPC. Several X-gal-positive, pro-SPC-positive cells are demonstrated (arrowheads) as well as a cluster of X-gal-positive, α-SMA-positive, and pro-SPC-negative cells (arrow). (C and D) The same field of a whole lung single-cell suspension from an injured lung stained by X-gal (D) then immunostained (C) for α-SMA and pro-SPC. An X-gal-positive, α-SMA-positive, pro-SPC-negative cell is demonstrated (arrow). (E and F) The same field of a whole lung single cell suspension from a triple transgenic mouse three weeks after AdTGF-β1, stained by X-gal (F), and immunostained (E) for vimentin. An X-gal- and vimentin-positive cell is indicated by arrow. (G) Quantification X-gal- and vimentin-positive single cells from of AdTGF-β1-injured and control lungs (n = 3 per group).