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TGF-β1-induced AEC apoptosis on Mg but not Fn. (A) Overlay of phase with TUNEL (green) and Dapi (blue) staining of AECs plated on Mg and treated with TGF-β1 (4 ng/ml) for 2 days. (B) Quantification of TUNEL-positive cells. (C and D) AECs from triple transgenic mice cultured on Mg then stimulated with TGF-β1 for 1 week, stained with X-gal (C) followed by immunostaining for α-SMA (D). A remaining X-gal-positive cell is shown (arrow) as well as several apoptotic remnants (arrow heads).
