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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: J Immunol. 2006 Jun 1;176(11):6640–6646. doi: 10.4049/jimmunol.176.11.6640

FIGURE 7.

FIGURE 7

Western blot analyses of effects of WT VPAC2 and SD VPAC2 signals on nuclear levels of transcription factors required for VIP enhancement of generation of IL-4 and the Th2 phenotype. The six lanes shown in each frame of this figure are analyses of nuclear proteins from transfectants expressing: WT VPAC2 without and with 10− 7 M VIP, respectively, SD VPAC2 plus WT VPAC2 (2:1) without and with 10−7 M VIP, and SD VPAC2 alone without and with 10−7 M VIP. A, c-Maf detected by rabbit anti-c-Maf IgG Ab. B, Jun B detected by rabbit anti-Jun B IgG Ab. C, Nuclear protein lamin B1 loading control marker detected by mouse anti-lamin B1 mAb.