FIGURE 8.
Effect of VIP on anti-CD3 stimulated increases in the nuclear level of active Jun B protein in D10G4.1 Th2 cell transfectants. Each column and bar represents the mean ± range of two studies in duplicate. Suspensions of 106 transfectants in 0.6 ml of RPMI 1640 with 10% FBS were preincubated 60 min without or with 10−7 M VIP and then incubated with 1 μg of anti-CD3 Ab for 4 h. Each number in parentheses is the mean increase (+) or decrease (−) in active nuclear Jun B attributable to VIP. The significance of differences in levels of active nuclear Jun B between samples without and with VIP was calculated by a paired t test; *, p < 0.01.