Table 1.
Quantitative GLP-2R RNA distribution in various rat tissues determined by RNase protection
| Tissue | F1 quantitation, copies per μg total RNA | β-Actin quantitation, copies per μg total RNA | GLP-2R/-actin, ratio |
|---|---|---|---|
| Jejunum | 11,900 | 15,500,000 | 76.8 × 10−5 |
| Duodenum | 9,150 | 85,700,000 | 10.7 × 10−5 |
| Ileum | 7,490 | 51,400,000 | 14.6 × 10−5 |
| Colon | 4,150 | 19,800,000 | 21.0 × 10−5 |
| Stomach | 1,530 | 23,600,000 | 6.48 × 10−5 |
| Brain | <600 | 40,600,000 | <1.48 × 10−5 |
| Heart | <600 | 6,600,000 | <9.09 × 10−5 |
| Kidney | <600 | 14,900,000 | <4.03 × 10−5 |
| Liver | <600 | 16,700,000 | <3.59 × 10−5 |
| Lung | <600 | 38,500,000 | <1.56 × 10−5 |
| Muscle | <600 | 4,600,000 | <13.0 × 10−5 |
| Spleen | <600 | 44,800,000 | <1.34 × 10−5 |
Total RNA (50 μg) from rat tissues or sense-strand cRNA standards was hybridized to radiolabeled antisense cRNA probes prepared in vitro from GLP-2R cDNA (F1) or actin cDNA. After RNase digestion as described in Methods and Materials, protected probe was precipitated, electrophoresed, and quantitated by PhosphorImage analysis relative to the standard curve obtained from sense-strand cRNA. RNA quantitation is expressed as copy number per μg of total RNA. GLP-2R RNA copy number was detectable to a lower limit of 30,000 copies per 50 μg sample, setting the limit of detection shown above for nongastrointestinal tissues.