Figure 8.

Orientation of cell divisions in mitotic region. (A) The orientation of mitotic spindles and/or metaphase plates was scored as along, across or oblique to the distal-proximal axis at each position. The majority of mitotic germ cells are found in the first 16 rows of the germ line; the likelihood of finding a germ cell in M-phase decreases more proximally. (B–E) Germ lines were stained with DAPI (white) or DAPI (white) and anti-tubulin (green). Arrowheads, distal ends. Germ cells in two different germ lines dividing across the distal-proximal axis at position 1, adjacent to the DTC (DAPI in B and anti-tubulin in C), oblique to the distal-proximal axis at position 1 (DAPI in D), or along the distal-proximal axis at position 5 (DAPI and anti-tubulin in E).