Figure 3.

GSLs are required for caveolar-mediated endocytosis of BODIPY-LacCer. CHO-K1 cells were pretreated with FB1, NB-DGJ, or PPPP for 48 h (see Materials and Methods). The internalization (5 min at 37°C) of BODIPY-LacCer, albumin, dextran, and IL-2R were then observed and quantified as in Figure 1. (A) Representative fluorescence micrographs for control, FB1 or NB-DGJ treated cells. Bar, 10 μm. (B) Quantitation of marker uptake. Results are expressed as percentage of marker uptake in untreated (control) cells. Values are the mean ± SD (n ≥ 50 cells for each marker).