FIG. 2.

Interactions between TPR1 and selected Gα proteins. HEK293T cells were transiently transfected with the FLAG-TPR1 construct either alone or with individual expression vectors encoding Gα16wt, Gαqwt, the short and long forms of Gαswt, Gαi2wt, Gα13wt, and Gαt-1wt. The cell homogenates were subjected to immunoprecipitation with an anti-FLAG agarose affinity gel and analyzed by Western blotting. The Western blot (WB) was probed for the respective Gα protein contents in the immunoprecipitates (IP) (top panels) and homogenates (H) (bottom panels). An equal amount of FLAG-TPR1 protein was precipitated in each sample (not shown). Data shown are representative of at least three independent experiments.