FIG. 5.

Implication of the TPR1 N terminus in Gα16-dependent enhancement of TPR1-Ras interaction. The full-length TPR1 (A) or the N-terminal deletion mutant (residues 113 to 292 deleted) (B) were transiently transfected in HEK293T cells, together with expression vectors coding for either Raswt or Gα16QL or both, as indicated. Immunoprecipitation (IP) was performed with anti-FLAG agarose affinity gels before Western blotting (WB) with anti-Ras (top panels), anti-Gα16 (second panels from top), and anti-FLAG (third panels from top) antibodies. Expression of Ras and Gα16 constructs was verified in the homogenates (H) (two bottom panels).