FIG. 9.

Interaction of Ha-Ras with the TPR-containing LGN protein. (A) Interaction between LGN and Gαi2 was confirmed in HEK293T cells that transiently expressed a FLAG-tagged full-length LGN protein or a C-terminal truncation mutant of LGN protein (400-stop). Cell lysates were subjected to immunoprecipitation using an anti-FLAG agarose affinity gel, and the immunoprecipitates (IP) were analyzed for Gαi2 by Western blotting (WB). (B) Same experiment as in panel A but with Raswt and RasG12V instead of Gαi2. The LGN-associated Ras was determined by Western blotting using an anti-Ras Ab (top panel). The FLAG-tagged proteins in the IP were shown with an anti-FLAG Ab and serve as loading controls (middle panels). Blots shown are representative of three experiments.