Table 1.

Cloned and sequenced PR-10 sequences from B. pendula. Overview of the individual clones of PR-10 sequences from B. pendula 'Schneverdinger Goldbirke', 'Tristis', and 'Long Trunk'. Different primers (BpI, BpII) were used. De number of cycles varied between 22 and 30. Confirmed sequences are found in multiple independent PCRs. Unique sequences differ at least by three base pairs from any other sequence from the same cultivar. The remaining sequences are either recombination artifacts or presumably result from base mis-incorporations. The number of alleles included for further analysis is also indicated.

Cultivar 'Long Trunk'
Primer combination no. of PCR cycles	BpI 22 cycles		BpII 24 cycles		BpI 30 cycles		No. of different alleles included
Confirmed sequences	43	(86%)	50	(69%)	24	(45%)	20
Unique sequences	2	(4%)	5	(7%)	5	(9%)	8
Recombination artifacts	1	(2%)	8	(11%)	18*1	(34%)
Base mis-incorporation artifacts	4	(8%)	9	(13%)	6	(11%)
Total no. of clones	50		72		53		28
Cultivar 'Tristis'
Primer combination no. of PCR cycles	BpI 22 cycles		BpII 24 cycles		BpI 30 cycles		No. of different alleles included
Confirmed sequences	17	(77%)	20	(53%)	26	(54%)	11
Unique sequences	1	(5%)	5	(13%)	0	-	4
Recombination artifacts	2	(9%)	2	(5%)	22*2	(46%)
Base mis-incorporation artifacts	2	(9%)	11	(29%)	0	-
Total no. of clones	22		38		48		15
Cultivar 'Schneverdinger Goldbirke'
Primer combination no. of PCR cycles	BpI 26 cycles		BpII 26 cycles		BpI 30 cycles		No. of different alleles included
Confirmed sequences	21	(53%)	13	(43%)	21	(43%)	10
Unique sequences	0	-	3	(10%)	3	(6%)	4
Recombination artifacts	12	(30%)	8	(27%)	15	(29%)
Base mis-incorporation artifacts	7	(18%)	6	(20%)	11	(22%)
Total no. of clones	40		30		51		14

^*1 one sequence had both a recombination and a base mis-incorporation artifact

^*2 two sequences had both a recombination and a base mis-incorporation artifact