Table 1.
Cultivar 'Long Trunk' | |||||||
Primer combination no. of PCR cycles |
BpI 22 cycles |
BpII 24 cycles |
BpI 30 cycles |
No. of different alleles included |
|||
Confirmed sequences | 43 | (86%) | 50 | (69%) | 24 | (45%) | 20 |
Unique sequences | 2 | (4%) | 5 | (7%) | 5 | (9%) | 8 |
Recombination artifacts | 1 | (2%) | 8 | (11%) | 18*1 | (34%) | |
Base mis-incorporation artifacts | 4 | (8%) | 9 | (13%) | 6 | (11%) | |
Total no. of clones | 50 | 72 | 53 | 28 | |||
Cultivar 'Tristis' | |||||||
Primer combination no. of PCR cycles |
BpI 22 cycles |
BpII 24 cycles |
BpI 30 cycles |
No. of different alleles included |
|||
Confirmed sequences | 17 | (77%) | 20 | (53%) | 26 | (54%) | 11 |
Unique sequences | 1 | (5%) | 5 | (13%) | 0 | - | 4 |
Recombination artifacts | 2 | (9%) | 2 | (5%) | 22*2 | (46%) | |
Base mis-incorporation artifacts | 2 | (9%) | 11 | (29%) | 0 | - | |
Total no. of clones | 22 | 38 | 48 | 15 | |||
Cultivar 'Schneverdinger Goldbirke' | |||||||
Primer combination no. of PCR cycles |
BpI 26 cycles |
BpII 26 cycles |
BpI 30 cycles |
No. of different alleles included |
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Confirmed sequences | 21 | (53%) | 13 | (43%) | 21 | (43%) | 10 |
Unique sequences | 0 | - | 3 | (10%) | 3 | (6%) | 4 |
Recombination artifacts | 12 | (30%) | 8 | (27%) | 15 | (29%) | |
Base mis-incorporation artifacts | 7 | (18%) | 6 | (20%) | 11 | (22%) | |
Total no. of clones | 40 | 30 | 51 | 14 |
*1 one sequence had both a recombination and a base mis-incorporation artifact
*2 two sequences had both a recombination and a base mis-incorporation artifact