Figure 3.

Standard curves of three methods form three different runs. (A) Normalized fluorescence ratio method standard curve: Normalized Fluorescence ratio was plotted against predefined ratio of allele YMDD/YIDD. (B) Baseline subtracted fluorescence ratio method standard curve: Baseline subtracted fluorescence ratio was plotted against predefined ratio of allele YMDD/YIDD (C): The value of Ct based method (2^-ΔCt) was plotted against predefined allele YMDM/YIDD. Ct values were determined by the second derivative maximum. Solid line, long dash line and dotted line respectively represent linear regression line of run 1, run 2 and run 3. Error bars indicate 1 SD for replicates.