Table 4.
intra-run and inter-run variations of three methods for three different runs
| allele ratio | Kf method | Baseline subtracted fluorescence ratio method | SDM method (2-ΔCt method) | |||||||||
| Run 1 | Run 2 | Run 3 | Combined | Run 1 | Run 2 | Run 3 | Combined | Run 1 | Run 2 | Run 3 | Combined | |
| intra C.V. | intra C.V. | intra C.V. | inter C.V. | intra C.V. | intra C.V. | intra C.V. | inter C.V. | intra C.V. | intra C.V. | intra C.V. | inter C.V. | |
| 90%:10% | 4.38% | 4.90% | 11.80% | 7.69% | 9.39% | 4.91% | 11.95% | 30.98% | 13.81% | 37.98% | 47.29% | 69.23% |
| 80%:20% | 9.18% | 16.15% | 5.45% | 12.54% | 5.30% | 9.11% | 6.45% | 21.53% | 26.72% | 20.39% | 3.30% | 23.96% |
| 70%:30% | 5.40% | 6.66% | 17.22% | 13.64% | 18.48% | 26.65% | 31.87% | 26.26% | 11.90% | 10.05% | 9.32% | 10.36% |
| 60%:40% | 2.99% | 4.44% | 6.11% | 10.91% | 4.41% | 10.11% | 12.22% | 11.55% | 3.52% | 7.41% | 24.43% | 15.06% |
| 50%:50% | 6.06% | 24.75% | 7.30% | 23.71% | 10.59% | 45.08% | 15.59% | 41.63% | 22.12% | 31.72% | 10.38% | 27.37% |
| 40%:60% | 28.91% | 27.05% | 7.77% | 20.61% | 33.55% | 34.65% | 13.09% | 34.48% | 38.09% | 13.30% | 8.56% | 24.53% |
| 30%:70% | 4.70% | 15.97% | 1.91% | 20.19% | 7.38% | 22.71% | 4.89% | 41.05% | 7.61% | 11.17% | 25.12% | 26.06% |
| 20%:80% | 29.02% | 18.71% | 3.67% | 17.82% | 36.42% | 10.93% | 6.57% | 30.18% | 13.72% | 13.23% | 42.29% | 28.82% |
| 10%:90% | 61.69% | 22.86% | 10.24% | 44.25% | 81.13% | 21.84% | 5.98% | 44.52% | 98.39% | 21.62% | 113.30% | 103.45% |