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. 2003 Jun;23(11):3813–3824. doi: 10.1128/MCB.23.11.3813-3824.2003

FIG. 1.

FIG. 1.

Duration and extent of ERK activation in cells subjected to various stresses. (A and C) Duration of ERK signal in cells subjected to various HS conditions. Cos-7 cells (A) or IMR90 cells (C) were exposed to the indicated HS conditions or left untreated (Cont.). Cells were harvested at the indicated time points after HS, and ERK activation was assayed by immunoblotting of cell lysates with anti-phospho-ERK antibody or tubulin to control for equal loading. (B and D) Activation of ERKs in cells subjected to various stresses. Cos-7 cells (B) or IMR90 cells (D) were subjected to HS (Cos-7 cells, 43°C for 30 min; IMR90 cells, 45°C for 30 min) or treated with 1 mM menadione for 30 min or 1 μM PDB for 7 min. ERK activation was assayed as in panels A and C.