Table 1.

Organisms identified by double matches of restriction enzyme/fluorescent label combinations

Enzyme/label combination	Fragment	Organism identified, species	strain; type culture collection number	number of plots (out of 24) where strain was detected
Bst-FAM × BstU-JOE	226/173	Clostridium botulinum	202F; ATCC 23387	5
		Clostridium butyricum	E.VI.3.6.1.; NCIMB 8082
		Clostridium butyricum	MMP3; DSM 2478
		Clostridium butyricum	ATCC 43755
	236/173	Bacillus subtilis		7
		unidentified species	16SX-1
		unidentified species	16SX-2
BstU-JOE × MNL-JOE	171/93	Ralstonia solanacearum	ACH 0732	24
	171/93	Piscirickettsia salmonis		5
	173/93	Pirellula sp. (uncultered)	clone 5H12	5
		Thermoanaerobacterium saccharolyticum	B6A-RI
	275/93	Ferribacterium limneticum	cda-1	3
		Escherichia coli chloroplast of Marchantia polymorpha
BstU-JOE × MNL-FAM	173/210	symbiont of Crithidia sp.		24
	275/205	unidentified strain from Gossenköllesee		3
	275/211	Azoarcus sp.	PH002	7
		Azoarcus sp.	CR23
		Azoarcus sp.	FL05
		unidentified species	clone A1–13
		unidentified species	cloneSJA-62
	275/217	Salmonella typhimurium	E10 NCTC 8391	15
	275/226	unidentified species	clone SJA-47	2
MNL-JOE × BstU-FAM	226/93	Clostridium algidicarnis	NCFB 2931 (T)	1
BstU-FAM × MNL-FAM	103/211	unidentified species	clone SJA-112	11
	219/426	Frankia sp.	L27	1
		Kibdelosporangium aridum subsp. aridum
		Mycobacterium asiaticum	N6IH; ATCC 25276
	221/129	Nocardia crassostreae	RB1	22
		Nocardia crassostreae	OB3
	221/183	Sulfobacillus disulfidooxidans	SD-11	12
		Kitasatospora paracochleata	14769
		Kitasatospora melanogena	JCM 3337
		Kitasatospora kifuense	JCM 9081
	236/138	Leuconostoc citreum	NCDO 2787	7
		Leuconostoc lactis	ATCC 19256
		Leuconostoc mesenteroides subsp. mesenteroides	ATCC 8293
	388/210	Oxalobacter formigenes	BLISS	12
	390/211	Pseudomonas sp.	B13	11
MNL-FAM × MNL-JOE	134/93	Clostridium perfringens	CPN50	2