Fig. 5.

Inhibition in adenoviral vector internalization in the presence of vector-specific fiber knob. MDBK cells in monolayer cultures were treated with 1, 5, or 25 μg/ml of the recombinant A) BAd3 (B3), B) HAd5 (H5), or C) PAd3 (P3) fiber knob followed by infection either with BAd-GFP, HAd-GFP, or PAd-GFP at an m.o.i. of 20 p.f.u. per cell. At 36 h post-infection, cells were harvested by trypsinization and the cells expressing GFP were sorted by flow cytometry. For positive controls, cell monolayers without any fiber knob treatment were infected similarly with BAd-GFP, HAd-GFP, or PAd-GFP. The transduction efficiencies of positive controls were taken as 100% for calculating the relative transduction of various treatment groups. The results are presented as the mean ± standard deviation of the percent relative transduction of minimum of three independent observations. *, P <0.05.