FIG. 1.

IL-13 induces 15-LO gene expression. Amplification plots of 15-LO (A) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (B) from real-time RT-PCR. Monocytes (5 × 10⁶) were treated with 500 pM IL-13 for different times. Total RNA was isolated, and RNA (1 μg) from each sample was used for real-time RT-PCR. The y axis shows the change in fluorescence intensity (ΔRn), and the x axis indicates the PCR cycle number. Each cycle represents a twofold difference in mRNA amounts. (C) This graph represents the induction of 15-LO mRNA expressed as induction as derived from the data presented in panel A. Data were collected from at least three independent experiments and are shown as means ± standard deviations. (D) Amplification plots showing inhibitory effects of actinomycin D on IL-13-induced 15-LO mRNA expression. The internal control glyceraldehyde-3-phosphate dehydrogenase plots (not shown) are similar to those in panel B. Data presented here are representative plots from two independent experiments.